BMRB: 11160

Solution structure of the HMG box of human Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog

Authors

Abe, H., Tochio, N., Miyamoto, K., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog

Entity

1. Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog (polymer, Thiol state: not present), 90 monomers, 10195.40 Da Detail

GSSGSSGNAQ RSTLKWEKEE ALGEMATVAP VLYTNINFPN LKEEFPDWTT RVKQIAKLWR KASSQERAPY VQKARDNRAA LRINKVQMSN

Formula weight

10195.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 92.9 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.9 % (991 of 1067)	92.0 % (518 of 563)	95.0 % (383 of 403)	89.1 % (90 of 101)
Backbone	91.5 % (487 of 532)	89.4 % (161 of 180)	93.2 % (248 of 266)	90.7 % (78 of 86)
Sidechain	94.4 % (586 of 621)	93.2 % (357 of 383)	97.3 % (217 of 223)	80.0 % (12 of 15)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (33 of 33)	100.0 % (3 of 3)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. HMG (high mobility group) box, UNP residues 1631-1713

GSSGSSGNAQ RSTLKWEKEE ALGEMATVAP VLYTNINFPN LKEEFPDWTT RVKQIAKLWR KASSQERAPY VQKARDNRAA LRINKVQMSN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.11mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	HMG (high mobility group) box, UNP residues 1631-1713	[U-13C; U-15N]	protein	1.11 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUK, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the HMG box of human Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog
Abe, H., Tochio, N., Miyamoto, K., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Related entities 1. Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog, : 1 : 9 entities Detail

Interaction partners 1. Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog, : 23 interactors Detail

Experiments performed 2 experiments Detail