BMRB: 11161

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain Tripartite motif protein 30

Authors

Abe, H., Miyamoto, K., Tochio, N., Yoneyama, M., Kigawa, T., Yokoyama, S.

Assembly

Tripartite motif-containing protein 30

Entity

1. RING-type, residues 6-85 (polymer, Thiol state: all other bound), 85 monomers, 9160.386 Da Detail

GSSGSSGMAS SVLEMIKEEV TCPICLELLK EPVSADCNHS FCRACITLNY ESNRNTDGKG NCPVCRVPYP FGNLKPNLHV ANIVE

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9291.204 Da

Max. entity weight

9160.386 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS22:SG	2:ZN1:ZN
2	na	sing	1:CYS25:SG	2:ZN1:ZN
3	na	sing	1:CYS37:SG	2:ZN1:ZN
4	na	sing	1:HIS39:ND1	2:ZN1:ZN
5	na	sing	1:CYS42:SG	2:ZN1:ZN
6	na	sing	1:CYS45:SG	2:ZN1:ZN
7	na	sing	1:CYS62:SG	2:ZN1:ZN
8	na	sing	1:CYS65:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 94.4 %, Completeness (bb): 92.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (882 of 934)	94.2 % (457 of 485)	95.0 % (344 of 362)	93.1 % (81 of 87)
Backbone	92.2 % (459 of 498)	91.2 % (155 of 170)	92.8 % (231 of 249)	92.4 % (73 of 79)
Sidechain	96.7 % (498 of 515)	95.9 % (302 of 315)	97.9 % (188 of 192)	100.0 % (8 of 8)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	97.7 % (84 of 86)	95.3 % (41 of 43)	100.0 % (43 of 43)

1. RING-type, residues 6-85

GSSGSSGMAS SVLEMIKEEV TCPICLELLK EPVSADCNHS FCRACITLNY ESNRNTDGKG NCPVCRVPYP FGNLKPNLHV ANIVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.13mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM Zncl2+1mM {IDA;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	RING-type, residues 6-85	[U-13C; U-15N]	protein	1.13 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	50 uM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECW, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain Tripartite motif protein 30
Abe, H., Miyamoto, K., Tochio, N., Yoneyama, M., Kigawa, T., Yokoyama, S.

Related entities 1. RING-type, residues 6-85, : 1 : 224 entities Detail

Interaction partners 1. RING-type, residues 6-85, : 2 interactors Detail

Experiments performed 2 experiments Detail