BMRB: 11162

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of RING finger protein 126

Authors

Abe, H., Miyamoto, K., Tochio, N., Kigawa, T., Yokoyama, S.

Assembly

RING finger protein 126

Entity

1. Zinc finger, C3HC4 type (RING finger) (polymer, Thiol state: all other bound), 78 monomers, 8181.964 Da Detail

GSSGSSGTEE HVGSGLECPV CKEDYALGES VRQLPCNHLF HDSCIVPWLE QHDSCPVCRK SLTGQNTATN PPGLTGVG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8312.782 Da

Max. entity weight

8181.964 Da

Entity Connection

na 8 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS36:SG	2:ZN1:ZN
4	na	sing	1:HIS38:ND1	2:ZN1:ZN
5	na	sing	1:HIS41:ND1	2:ZN1:ZN
6	na	sing	1:CYS44:SG	2:ZN1:ZN
7	na	sing	1:CYS55:SG	2:ZN1:ZN
8	na	sing	1:CYS58:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 96.5 %, Completeness (bb): 95.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (791 of 820)	96.5 % (408 of 423)	96.9 % (308 of 318)	94.9 % (75 of 79)
Backbone	95.0 % (433 of 456)	94.4 % (151 of 160)	95.5 % (214 of 224)	94.4 % (68 of 72)
Sidechain	98.1 % (424 of 432)	97.7 % (257 of 263)	98.8 % (160 of 162)	100.0 % (7 of 7)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (22 of 22)	100.0 % (1 of 1)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. Zinc finger, C3HC4 type (RING finger)

GSSGSSGTEE HVGSGLECPV CKEDYALGES VRQLPCNHLF HDSCIVPWLE QHDSCPVCRK SLTGQNTATN PPGLTGVG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.06mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3 {;0.05mM} ZnCl2+1mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Zinc finger, C3HC4 type (RING finger)	[U-13C; U-15N]	protein	1.06 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2ECT, Strand ID: A Detail

---

Release date

2011-05-04

Citation

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of RING finger protein 126
Abe, H., Miyamoto, K., Tochio, N., Kigawa, T., Yokoyama, S.

Related entities 1. Zinc finger, C3HC4 type (RING finger), : 1 : 3 : 53 entities Detail

Interaction partners 1. Zinc finger, C3HC4 type (RING finger), : 29 interactors Detail

Experiments performed 2 experiments Detail