BMRB: 11163

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of Tripartite motif-containing protein 5

Authors

Abe, H., Miyamoto, K., Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

Tripartite motif-containing protein 5

Entity

1. Zinc finger, C3HC4 type (RING finger) (polymer, Thiol state: all other bound), 85 monomers, 9104.367 Da Detail

GSSGSSGMAS GILVNVKEEV TCPICLELLT QPLSLDCGHS FCQACLTANH KKSMLDKGES SCPVCRISYQ PENIRPNRHV ANIVE

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9235.186 Da

Max. entity weight

9104.367 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS22:SG	2:ZN1:ZN
2	na	sing	1:CYS25:SG	2:ZN1:ZN
3	na	sing	1:CYS37:SG	2:ZN1:ZN
4	na	sing	1:HIS39:ND1	2:ZN1:ZN
5	na	sing	1:CYS42:SG	2:ZN1:ZN
6	na	sing	1:CYS45:SG	2:ZN1:ZN
7	na	sing	1:CYS62:SG	2:ZN1:ZN
8	na	sing	1:CYS65:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 92.5 %, Completeness (bb): 89.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.5 % (861 of 931)	93.0 % (450 of 484)	93.0 % (334 of 359)	87.5 % (77 of 88)
Backbone	89.0 % (445 of 500)	88.3 % (151 of 171)	90.4 % (225 of 249)	86.3 % (69 of 80)
Sidechain	96.1 % (490 of 510)	95.5 % (299 of 313)	96.8 % (183 of 189)	100.0 % (8 of 8)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	96.7 % (87 of 90)	95.6 % (43 of 45)	97.8 % (44 of 45)

1. Zinc finger, C3HC4 type (RING finger)

GSSGSSGMAS GILVNVKEEV TCPICLELLT QPLSLDCGHS FCQACLTANH KKSMLDKGES SCPVCRISYQ PENIRPNRHV ANIVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, pH 7.0 (±0.05), Details 1.25mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3 {;0.05mM} Zncl2+1mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Zinc finger, C3HC4 type (RING finger)	[U-13C; U-15N]	protein	1.25 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.2 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECV, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of Tripartite motif-containing protein 5
Abe, H., Miyamoto, K., Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. Zinc finger, C3HC4 type (RING finger), : 1 : 4 : 216 entities Detail

Interaction partners 1. Zinc finger, C3HC4 type (RING finger), : 22 interactors Detail

Experiments performed 2 experiments Detail