BMRB: 11164

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of TNF receptor-associated factor 3

Authors

Abe, H., Miyamoto, K., Tochio, N., Yoneyama, M., Kigawa, T., Yokoyama, S.

Assembly

TNF receptor-associated factor 3

Entity

1. RING-type, residues 8-66 (polymer, Thiol state: free and other bound), 66 monomers, 7125.164 Da Detail

GSSGSSGFVK TVEDKYKCEK CHLVLCSPKQ TECGHRFCES CMAALLSSSS PKCTACQESI VKDKVF

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7255.982 Da

Max. entity weight

7125.164 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS33:SG	2:ZN1:ZN
4	na	sing	1:HIS35:ND1	2:ZN1:ZN
5	na	sing	1:CYS38:SG	2:ZN1:ZN
6	na	sing	1:CYS41:SG	2:ZN1:ZN
7	na	sing	1:CYS53:SG	2:ZN1:ZN
8	na	sing	1:CYS56:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 91.6 %, Completeness (bb): 89.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (669 of 730)	91.1 % (346 of 380)	93.0 % (264 of 284)	89.4 % (59 of 66)
Backbone	89.3 % (350 of 392)	86.6 % (116 of 134)	91.2 % (177 of 194)	89.1 % (57 of 64)
Sidechain	94.2 % (377 of 400)	93.5 % (230 of 246)	95.4 % (145 of 152)	100.0 % (2 of 2)
Aromatic	82.6 % (38 of 46)	78.3 % (18 of 23)	87.0 % (20 of 23)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. RING-type, residues 8-66

GSSGSSGFVK TVEDKYKCEK CHLVLCSPKQ TECGHRFCES CMAALLSSSS PKCTACQESI VKDKVF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.10mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM Zncl2+1mM {IDA;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	RING-type, residues 8-66	[U-13C; U-15N]	protein	1.10 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	50 uM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.36 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECY, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of TNF receptor-associated factor 3
Abe, H., Miyamoto, K., Tochio, N., Yoneyama, M., Kigawa, T., Yokoyama, S.

Related entities 1. RING-type, residues 8-66, : 1 : 2 : 106 entities Detail

Interaction partners 1. RING-type, residues 8-66, : 41 interactors Detail

Experiments performed 2 experiments Detail