BMRB: 11165

Solution structure of the C2H2 type zinc finger (region 329-359) of human Zinc finger protein 268

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2 domain (polymer, Thiol state: all other bound), 44 monomers, 4684.019 Da Detail

GSSGSSGEKL HECSECRKTF SFHSQLVIHQ RIHTGENPSG PSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4749.428 Da

Max. entity weight

4684.019 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.4 %, Completeness: 86.7 %, Completeness (bb): 80.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.7 % (409 of 472)	87.9 % (217 of 247)	86.7 % (156 of 180)	80.0 % (36 of 45)
Backbone	80.0 % (208 of 260)	79.3 % (73 of 92)	81.0 % (102 of 126)	78.6 % (33 of 42)
Sidechain	94.0 % (235 of 250)	92.9 % (144 of 155)	95.7 % (88 of 92)	100.0 % (3 of 3)
Aromatic	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	83.3 % (20 of 24)	83.3 % (10 of 12)	83.3 % (10 of 12)

1. zf-C2H2 domain

GSSGSSGEKL HECSECRKTF SFHSQLVIHQ RIHTGENPSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details about 1.0mM sample U-15N, {13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 0.05mM {ZnCl2;} 1mM {IDA;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	ZnCl2	natural abundance	salt	0.05 mM
5	IDA	natural abundance	salt	1 mM
6	d-DTT	natural abundance	salt	1 mM
7	NaN3	natural abundance	salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.23 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.23 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EOI, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the C2H2 type zinc finger (region 329-359) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 264 entities Detail

Experiments performed 2 experiments Detail