BMRB: 11166

Solution structure of the C2H2 type zinc finger (region 581-609) of human Zinc finger protein 268

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 268

Entity

1. zf-C2H2 domain (polymer, Thiol state: all other bound), 42 monomers, 4282.598 Da Detail

GSSGSSGEKP YECTDCGKAF GLKSQLIIHQ RTHTGESGPS SG

2. ZN (non-polymer), 65.409 Da

Total weight

4348.0073 Da

Max. entity weight

4282.598 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS16:SG	2:ZN1:ZN
3	na	sing	1:HIS29:NE2	2:ZN1:ZN
4	na	sing	1:HIS33:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.0 %, Completeness: 86.0 %, Completeness (bb): 79.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.0 % (374 of 435)	86.3 % (196 of 227)	87.3 % (145 of 166)	78.6 % (33 of 42)
Backbone	79.8 % (198 of 248)	77.8 % (70 of 90)	82.2 % (97 of 118)	77.5 % (31 of 40)
Sidechain	92.8 % (205 of 221)	92.0 % (126 of 137)	93.9 % (77 of 82)	100.0 % (2 of 2)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)

1. zf-C2H2 domain

GSSGSSGEKP YECTDCGKAF GLKSQLIIHQ RTHTGESGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.0mM sample U-15N, {13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 0.05mM {ZnCl2;} 1mM {IDA;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	ZnCl2	natural abundance	salt	0.05 mM
5	IDA	natural abundance	salt	1 mM
6	d-DTT	natural abundance	salt	1 mM
7	NaN3	natural abundance	salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EOL, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the C2H2 type zinc finger (region 581-609) of human Zinc finger protein 268
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 181 entities Detail

Experiments performed 2 experiments Detail