BMRB: 11167

Solution structure of the C2H2 type zinc finger (region 508-540) of human Zinc finger protein 347

Authors

Abe, H., Saito, K., Tochio, N., Tomizawa, T., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 347

Entity

1. zf-C2H2, UNP residues 508-540 (polymer, Thiol state: all other bound), 46 monomers, 4715.076 Da Detail

GSSGSSGTGE KPYKCNECGK VFTQNSHLAN HQRIHTGVKP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4780.4854 Da

Max. entity weight

4715.076 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.3 %, Completeness: 78.5 %, Completeness (bb): 70.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.5 % (380 of 484)	78.7 % (200 of 254)	80.2 % (146 of 182)	70.8 % (34 of 48)
Backbone	70.0 % (189 of 270)	64.9 % (63 of 97)	74.6 % (97 of 130)	67.4 % (29 of 43)
Sidechain	88.1 % (222 of 252)	87.3 % (137 of 157)	88.9 % (80 of 90)	100.0 % (5 of 5)
Aromatic	76.7 % (23 of 30)	73.3 % (11 of 15)	80.0 % (12 of 15)
Methyl	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)

1. zf-C2H2, UNP residues 508-540

GSSGSSGTGE KPYKCNECGK VFTQNSHLAN HQRIHTGVKP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details about 1.0mM sample {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 0.05mM {ZnCl2;} 1mM {IDA;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2, UNP residues 508-540	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	ZnCl2	natural abundance	salt	0.05 mM
5	IDA	natural abundance	salt	1 mM
6	d-DTT	natural abundance	salt	1 mM
7	NaN3	natural abundance	salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EOE, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the C2H2 type zinc finger (region 508-540) of human Zinc finger protein 347
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, UNP residues 508-540, : 1 : 139 entities Detail

Experiments performed 2 experiments Detail