Solution structure of the C2H2 type zinc finger (region 435-467) of human Zinc finger protein 484
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.8 % (396 of 484) | 83.1 % (212 of 255) | 82.5 % (151 of 183) | 71.7 % (33 of 46) |
Backbone | 73.0 % (197 of 270) | 71.1 % (69 of 97) | 75.4 % (98 of 130) | 69.8 % (30 of 43) |
Sidechain | 91.3 % (230 of 252) | 90.5 % (143 of 158) | 92.3 % (84 of 91) | 100.0 % (3 of 3) |
Aromatic | 100.0 % (30 of 30) | 100.0 % (15 of 15) | 100.0 % (15 of 15) | |
Methyl | 100.0 % (20 of 20) | 100.0 % (10 of 10) | 100.0 % (10 of 10) |
1. zf-C2H2, UNP residues 435-467
GSSGSSGTGE KPYECSDCGK SFIKKSQLHV HQRIHTGENP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details about 1.0mM sample {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 0.05mM {ZnCl2;} 1mM {IDA;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2, UNP residues 435-467 | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | ZnCl2 | natural abundance | salt | 0.05 mM |
5 | IDA | natural abundance | salt | 1 mM |
6 | d-DTT | natural abundance | salt | 1 mM |
7 | NaN3 | natural abundance | salt | 0.02 % |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |