BMRB: 11173

Solution Structure of the First UBA Domain in the Human Ubiquitin Specific Protease 5 (Isopeptidase 5)

Authors

Zhao, C., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Ubiquitin carboxyl-terminal hydrolase 5

Entity

1. Ubiquitin carboxyl-terminal hydrolase 5 (polymer, Thiol state: all free), 74 monomers, 7497.219 Da Detail

GSSGSSGLDE SVIIQLVEMG FPMDACRKAV YYTGNSGAEA AMNWVMSHMD DPDFANPLIL PGSSGPGSSG PSSG

Formula weight

7497.219 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.8 %, Completeness: 85.0 %, Completeness (bb): 81.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.0 % (646 of 760)	83.8 % (327 of 390)	86.5 % (257 of 297)	84.9 % (62 of 73)
Backbone	81.5 % (352 of 432)	78.4 % (120 of 153)	82.9 % (175 of 211)	83.8 % (57 of 68)
Sidechain	89.3 % (349 of 391)	87.3 % (207 of 237)	91.9 % (137 of 149)	100.0 % (5 of 5)
Aromatic	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (25 of 25)	100.0 % (1 of 1)
Methyl	96.6 % (56 of 58)	93.1 % (27 of 29)	100.0 % (29 of 29)

1. UBA domain

GSSGSSGLDE SVIIQLVEMG FPMDACRKAV YYTGNSGAEA AMNWVMSHMD DPDFANPLIL PGSSGPGSSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296.0 (±0.1) K, pH 7.0 (±0.05), Details 1.05mM UBA domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UBA domain	[U-13C; U-15N]	protein	1.05 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O	natural abundance	solvent	90 %
7	D2O	natural abundance	solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAG, Strand ID: A Detail

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Release date

2010-06-15

Citation

Solution Structure of the First UBA Domain in the Human Ubiquitin Specific Protease 5 (Isopeptidase 5)
Zhao, C., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Ubiquitin carboxyl-terminal hydrolase 5, : 1 : 38 entities Detail

Interaction partners 1. Ubiquitin carboxyl-terminal hydrolase 5, : 14 interactors Detail

Experiments performed 2 experiments Detail