BMRB: 11176

Solution Structure of RNA aptamer against AML1 Runt domain

Authors

Nomura, Y., Fujiwara, K., Chiba, M., Fukunaga, J., Tanaka, Y., Iibuchi, H., Tanaka, T., Nakamura, Y., Kawai, G., Kozu, T., Sakamoto, T.

Assembly

AML22

Entity

1. AML22 (polymer, Thiol state: not present), 22 monomers, 7133.285 Da Detail

GGACCCACCA CGGCGAGGUC CA

Formula weight

7133.285 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 53.8 %, Completeness (bb): 51.5 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	53.8 % (105 of 195)	53.8 % (105 of 195)
Suger, PO4	51.5 % (68 of 132)	51.5 % (68 of 132)
Nucleobase	58.7 % (37 of 63)	58.7 % (37 of 63)
Aromatic	82.2 % (37 of 45)	82.2 % (37 of 45)

1. AML22

GGACCCACCA CGGCGAGGUC CA

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	AML22	natural abundance	RNA	0.6 mM
2	sodium phosphate	natural abundance	salt	10 mM
3	D2O	natural abundance	solvent	100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	AML22	natural abundance	RNA	0.6 mM
8	sodium phosphate	natural abundance	salt	10 mM
9	H2O	natural abundance	solvent	90 %
10	D2O	natural abundance	solvent	10 %

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Release date

2015-06-22

Citation

A novel high affinity RNA motif that mimics DNA in AML1 Runt domain binding
Nomura, Y., Fukunaga, J., Tanaka, Y., Fujiwara, K., Chiba, M., Iibuchi, H., Tanaka, T., Nakamura, Y., Kawai, G., Sakamoto, T., Kozu, T.

Related entities 1. AML22, : 1 entities Detail

Experiments performed 9 experiments Detail