BMRB: 11180

Solution structure of the first SH3 domain of KIAA0318 protein

Authors

Inoue, K., Hayashi, F., Yokoyama, S.

Assembly

RIM binding protein 2

Entity

1. RIM binding protein 2 (polymer, Thiol state: all free), 96 monomers, 10338.01 Da Detail

GSSGSSGTSK QRYSGKVHLC VARYSYNPFD GPNENPEAEL PLTAGKYLYV YGDMDEDGFY EGELLDGQRG LVPSNFVDFV QDNESRLAST SGPSSG

Formula weight

10338.01 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.6 %, Completeness (bb): 99.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.6 % (1041 of 1045)	99.3 % (538 of 542)	100.0 % (405 of 405)	100.0 % (98 of 98)
Backbone	99.6 % (562 of 564)	99.0 % (197 of 199)	100.0 % (275 of 275)	100.0 % (90 of 90)
Sidechain	99.6 % (562 of 564)	99.4 % (341 of 343)	100.0 % (213 of 213)	100.0 % (8 of 8)
Aromatic	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. SH3 domain

GSSGSSGTSK QRYSGKVHLC VARYSYNPFD GPNENPEAEL PLTAGKYLYV YGDMDEDGFY EGELLDGQRG LVPSNFVDFV QDNESRLAST SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 1.26mM 13C, 15N-labeled {protein;} 20mM {PiNa;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.26 mM
2	PiNa	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WIE, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the first SH3 domain of KIAA0318 protein
Inoue, K., Hayashi, F., Yokoyama, S.

Related entities 1. RIM binding protein 2, : 1 : 47 entities Detail

Interaction partners 1. RIM binding protein 2, : 3 interactors Detail

Experiments performed 2 experiments Detail