BMRB: 11181

Solution structure of the fourth fn3 domain of KIAA1496 protein

Authors

Inoue, K., Nagashima, T., Tochio, N., Kigawa, T., Hayashi, F., Yokoyama, S.

Assembly

KIAA1496 protein

Entity

1. KIAA1496 protein (polymer, Thiol state: not present), 117 monomers, 12503.64 Da Detail

GSSGSSGTVN VTTKKTPPSQ PPGNVVWNAT DTKVLLNWEQ VKAMENESEV TGYKVFYRTS SQNNVQVLNT NKTSAELVLP IKEDYIIEVK ATTDGGDGTS SEQIRIPRIT SSGPSSG

Formula weight

12503.64 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 95.4 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.4 % (1234 of 1293)	96.1 % (643 of 669)	95.6 % (476 of 498)	91.3 % (115 of 126)
Backbone	93.0 % (640 of 688)	93.7 % (222 of 237)	93.5 % (319 of 341)	90.0 % (99 of 110)
Sidechain	97.8 % (696 of 712)	97.5 % (421 of 432)	98.1 % (259 of 264)	100.0 % (16 of 16)
Aromatic	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (27 of 27)	100.0 % (2 of 2)
Methyl	99.2 % (127 of 128)	100.0 % (64 of 64)	98.4 % (63 of 64)

1. fn3 domain

GSSGSSGTVN VTTKKTPPSQ PPGNVVWNAT DTKVLLNWEQ VKAMENESEV TGYKVFYRTS SQNNVQVLNT NKTSAELVLP IKEDYIIEVK ATTDGGDGTS SEQIRIPRIT SSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.98mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	0.98 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.7 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJ3, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the fourth fn3 domain of KIAA1496 protein
Inoue, K., Nagashima, T., Tochio, N., Kigawa, T., Hayashi, F., Yokoyama, S.

Related entities 1. KIAA1496 protein, : 1 : 223 entities Detail

Interaction partners 1. KIAA1496 protein, : 4 interactors Detail

Experiments performed 2 experiments Detail