BMRB: 11183

Solution structure of the FNIII domain of human RIM-binding protein 2

Authors

Inoue, K., Hayashi, F., Yokoyama, S.

Assembly

RIM binding protein 2

Entity

1. RIM binding protein 2 (polymer, Thiol state: not present), 130 monomers, 13009.44 Da Detail

GSSGSSGVEF STLPAGPPAP PQDVTVQAGV TPATIRVSWR PPVLTPTGLS NGANVTGYGV YAKGQRVAEV IFPTADSTAV ELVRLRSLEA KGVTVRTLSA QGESVDSAVA AVPPELLVPP TPHPSGPSSG

Formula weight

13009.44 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 94.2 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.2 % (1279 of 1358)	94.2 % (654 of 694)	94.5 % (514 of 544)	92.5 % (111 of 120)
Backbone	92.4 % (689 of 746)	92.2 % (237 of 257)	92.6 % (348 of 376)	92.0 % (104 of 113)
Sidechain	96.3 % (701 of 728)	95.4 % (417 of 437)	97.5 % (277 of 284)	100.0 % (7 of 7)
Aromatic	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (25 of 25)	100.0 % (1 of 1)
Methyl	100.0 % (168 of 168)	100.0 % (84 of 84)	100.0 % (84 of 84)

1. fn3 domain

GSSGSSGVEF STLPAGPPAP PQDVTVQAGV TPATIRVSWR PPVLTPTGLS NGANVTGYGV YAKGQRVAEV IFPTADSTAV ELVRLRSLEA KGVTVRTLSA QGESVDSAVA AVPPELLVPP TPHPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.09mM U-15N, 13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1.09 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CSP, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the FNIII domain of human RIM-binding protein 2
Inoue, K., Hayashi, F., Yokoyama, S.

Related entities 1. RIM binding protein 2, : 1 : 2 : 40 entities Detail

Interaction partners 1. RIM binding protein 2, : 3 interactors Detail

Experiments performed 2 experiments Detail