BMRB: 11184

Solution structure of the second SH3 domain of human RIM-binding protein 2

Authors

Inoue, K., Hayashi, F., Yokoyama, S.

Assembly

RIM binding protein 2

Entity

1. RIM binding protein 2 (polymer, Thiol state: all free), 97 monomers, 10337.22 Da Detail

GSSGSSGTDP GAEELPARIF VALFDYDPLT MSPNPDAAEE ELPFKEGQII KVYGDKDADG FYRGETCARL GLIPCNMVSE IQADDEEMMD QSGPSSG

Formula weight

10337.22 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 92.7 %, Completeness (bb): 92.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (966 of 1042)	91.3 % (495 of 542)	94.1 % (382 of 406)	94.7 % (89 of 94)
Backbone	92.2 % (522 of 566)	89.8 % (177 of 197)	93.2 % (261 of 280)	94.4 % (84 of 89)
Sidechain	93.8 % (527 of 562)	92.2 % (318 of 345)	96.2 % (204 of 212)	100.0 % (5 of 5)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)
Methyl	96.2 % (75 of 78)	92.3 % (36 of 39)	100.0 % (39 of 39)

1. SH3 domain

GSSGSSGTDP GAEELPARIF VALFDYDPLT MSPNPDAAEE ELPFKEGQII KVYGDKDADG FYRGETCARL GLIPCNMVSE IQADDEEMMD QSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.01mM U-15N, 13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.01 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CSQ, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the second SH3 domain of human RIM-binding protein 2
Inoue, K., Hayashi, F., Yokoyama, S.

Related entities 1. RIM binding protein 2, : 1 : 6 : 63 entities Detail

Interaction partners 1. RIM binding protein 2, : 3 interactors Detail

Experiments performed 2 experiments Detail