BMRB: 11185

Solution structure of the third SH3 domain of human RIM-binding protein 2

Authors

Inoue, K., Hayashi, F., Yokoyama, S.

Assembly

RIM binding protein 2

Entity

1. RIM binding protein 2 (polymer, Thiol state: all free), 76 monomers, 8116.663 Da Detail

GSSGSSGRRM VALYDYDPRE SSPNVDVEAE LTFCTGDIIT VFGEIDEDGF YYGELNGQKG LVPSNFLEEV SGPSSG

Formula weight

8116.663 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 93.3 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.3 % (760 of 815)	93.6 % (392 of 419)	93.8 % (300 of 320)	89.5 % (68 of 76)
Backbone	89.7 % (402 of 448)	88.7 % (141 of 159)	90.8 % (197 of 217)	88.9 % (64 of 72)
Sidechain	97.0 % (419 of 432)	96.5 % (251 of 260)	97.6 % (164 of 168)	100.0 % (4 of 4)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. SH3 domain

GSSGSSGRRM VALYDYDPRE SSPNVDVEAE LTFCTGDIIT VFGEIDEDGF YYGELNGQKG LVPSNFLEEV SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.08mM U-15N, 13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.08 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.32 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.32 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.21 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CSI, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the third SH3 domain of human RIM-binding protein 2
Inoue, K., Hayashi, F., Yokoyama, S.

Related entities 1. RIM binding protein 2, : 1 : 7 : 250 entities Detail

Interaction partners 1. RIM binding protein 2, : 3 interactors Detail

Experiments performed 2 experiments Detail