BMRB: 11186

Solution structure of the fifth PDZ domain of InaD-like protein

Authors

Inoue, K., Hayashi, F., Yokoyama, S.

Assembly

InaD-like protein

Entity

1. InaD-like protein (polymer, Thiol state: all free), 108 monomers, 11162.61 Da Detail

GSSGSSGELA LWSPEVKIVE LVKDCKGLGF SILDYQDPLD PTRSVIVIRS LVADGVAERS GGLLPGDRLV SVNEYCLDNT SLAEAVEILK AVPPGLVHLG ICSGPSSG

Formula weight

11162.61 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 97.2 %, Completeness (bb): 95.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.2 % (1131 of 1163)	96.8 % (577 of 596)	98.5 % (455 of 462)	94.3 % (99 of 105)
Backbone	95.7 % (607 of 634)	95.0 % (211 of 222)	96.8 % (301 of 311)	94.1 % (95 of 101)
Sidechain	98.1 % (612 of 624)	97.6 % (365 of 374)	98.8 % (243 of 246)	100.0 % (4 of 4)
Aromatic	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (20 of 20)	100.0 % (1 of 1)
Methyl	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (74 of 74)

1. PDZ domain

GSSGSSGELA LWSPEVKIVE LVKDCKGLGF SILDYQDPLD PTRSVIVIRS LVADGVAERS GGLLPGDRLV SVNEYCLDNT SLAEAVEILK AVPPGLVHLG ICSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.43mM U-15N, 13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.43 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D92, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the fifth PDZ domain of InaD-like protein
Inoue, K., Hayashi, F., Yokoyama, S.

Related entities 1. InaD-like protein, : 1 : 2 : 134 entities Detail

Interaction partners 1. InaD-like protein, : 42 interactors Detail

Experiments performed 2 experiments Detail