BMRB: 11187

Solution structure of the 7th PDZ domain of InaD-like protein

Authors

Inoue, K., Nagashima, T., Izumi, K., Hayashi, F., Yokoyama, S.

Assembly

InaD-like protein

Entity

1. InaD-like protein (polymer, Thiol state: not present), 124 monomers, 13250.74 Da Detail

GSSGSSGDAF TDQKIRQRYA DLPGELHIIE LEKDKNGLGL SLAGNKDRSR MSIFVVGINP EGPAAADGRM RIGDELLEIN NQILYGRSHQ NASAIIKTAP SKVKLVFIRN EDAVNQMASG PSSG

Formula weight

13250.74 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 95.1 %, Completeness (bb): 92.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (1311 of 1379)	95.2 % (687 of 722)	95.8 % (503 of 525)	91.7 % (121 of 132)
Backbone	92.9 % (682 of 734)	92.6 % (238 of 257)	93.9 % (336 of 358)	90.8 % (108 of 119)
Sidechain	97.4 % (735 of 755)	96.6 % (449 of 465)	98.6 % (273 of 277)	100.0 % (13 of 13)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)
Methyl	100.0 % (130 of 130)	100.0 % (65 of 65)	100.0 % (65 of 65)

1. PDZ domain

GSSGSSGDAF TDQKIRQRYA DLPGELHIIE LEKDKNGLGL SLAGNKDRSR MSIFVVGINP EGPAAADGRM RIGDELLEIN NQILYGRSHQ NASAIIKTAP SKVKLVFIRN EDAVNQMASG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.28mM U-15N,13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.28 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAZ, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the 7th PDZ domain of InaD-like protein
Inoue, K., Nagashima, T., Izumi, K., Hayashi, F., Yokoyama, S.

Related entities 1. InaD-like protein, : 1 : 2 : 59 entities Detail

Interaction partners 1. InaD-like protein, : 42 interactors Detail

Experiments performed 2 experiments Detail