BMRB: 11188

Solution structure of the first PDZ domain of InaD-like protein

Authors

Inoue, K., Kurosaki, C., Hayashi, F., Yokoyama, S.

Assembly

InaD-like protein

Entity

1. InaD-like protein (polymer, Thiol state: not present), 128 monomers, 13681.01 Da Detail

GSSGSSGLGN EDFNSVIQQM AQGRQIEYID IERPSTGGLG FSVVALRSQN LGKVDIFVKD VQPGSVADRD QRLKENDQIL AINHTPLDQN ISHQQAIALL QQTTGSLRLI VAREPVHTKS STSGPSSG

Formula weight

13681.01 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 92.9 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.9 % (1320 of 1421)	91.8 % (679 of 740)	95.2 % (513 of 539)	90.1 % (128 of 142)
Backbone	91.0 % (690 of 758)	88.6 % (234 of 264)	93.5 % (347 of 371)	88.6 % (109 of 123)
Sidechain	95.2 % (741 of 778)	93.5 % (445 of 476)	97.9 % (277 of 283)	100.0 % (19 of 19)
Aromatic	96.0 % (48 of 50)	92.0 % (23 of 25)	100.0 % (25 of 25)
Methyl	98.6 % (144 of 146)	97.3 % (71 of 73)	100.0 % (73 of 73)

1. PDZ domain

GSSGSSGLGN EDFNSVIQQM AQGRQIEYID IERPSTGGLG FSVVALRSQN LGKVDIFVKD VQPGSVADRD QRLKENDQIL AINHTPLDQN ISHQQAIALL QQTTGSLRLI VAREPVHTKS STSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.37mM U-15N,13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.37 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DB5, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the first PDZ domain of InaD-like protein
Inoue, K., Kurosaki, C., Hayashi, F., Yokoyama, S.

Related entities 1. InaD-like protein, : 1 : 2 : 61 entities Detail

Interaction partners 1. InaD-like protein, : 43 interactors Detail

Experiments performed 2 experiments Detail