BMRB: 11190

Solution structure of the third PDZ domain of human InaD-like protein

Authors

Inoue, K., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

InaD-like protein

Entity

1. InaD-like protein (polymer, Thiol state: not present), 129 monomers, 13291.46 Da Detail

GSSGSSGGSD SSLFETYNVE LVRKDGQSLG IRIVGYVGTS HTGEASGIYV KSVIPGSAAY HNGHIQVNDK IVAVDGVNIQ GFANHDVVEV LRNAGQVVHL TLVRRKTSSS TSPLEPPSDR GTVSGPSSG

Formula weight

13291.46 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 94.7 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (1287 of 1359)	94.2 % (654 of 694)	95.9 % (509 of 531)	92.5 % (124 of 134)
Backbone	93.2 % (712 of 764)	92.6 % (251 of 271)	94.0 % (347 of 369)	91.9 % (114 of 124)
Sidechain	96.5 % (681 of 706)	95.3 % (403 of 423)	98.2 % (268 of 273)	100.0 % (10 of 10)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)
Methyl	98.0 % (147 of 150)	96.0 % (72 of 75)	100.0 % (75 of 75)

1. PDZ domain

GSSGSSGGSD SSLFETYNVE LVRKDGQSLG IRIVGYVGTS HTGEASGIYV KSVIPGSAAY HNGHIQVNDK IVAVDGVNIQ GFANHDVVEV LRNAGQVVHL TLVRRKTSSS TSPLEPPSDR GTVSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.26mM U-15N,13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.26 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMZ, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the third PDZ domain of human InaD-like protein
Inoue, K., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. InaD-like protein, : 1 : 94 entities Detail

Interaction partners 1. InaD-like protein, : 42 interactors Detail

Experiments performed 2 experiments Detail