BMRB: 11195

Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11

Authors

Inoue, K., Suetake, T., Hayashi, F., Yokoyama, S.

Assembly

Rho guanine nucleotide exchange factor 11

Entity

1. Rho guanine nucleotide exchange factor 11 (polymer, Thiol state: all free), 93 monomers, 9429.691 Da Detail

GSSGSSGVQR CVIIQKDQHG FGFTVSGDRI VLVQSVRPGG AAMKAGVKEG DRIIKVNGTM VTNSSHLEVV KLIKSGAYVA LTLLGSSSGP SSG

Formula weight

9429.691 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 91.6 %, Completeness (bb): 88.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (910 of 993)	91.0 % (464 of 510)	93.5 % (361 of 386)	87.6 % (85 of 97)
Backbone	88.4 % (490 of 554)	86.4 % (172 of 199)	90.5 % (239 of 264)	86.8 % (79 of 91)
Sidechain	95.4 % (493 of 517)	93.9 % (292 of 311)	97.5 % (195 of 200)	100.0 % (6 of 6)
Aromatic	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. PDZ domain

GSSGSSGVQR CVIIQKDQHG FGFTVSGDRI VLVQSVRPGG AAMKAGVKEG DRIIKVNGTM VTNSSHLEVV KLIKSGAYVA LTLLGSSSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.20mM U-15N, 13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.20 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.28 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DLS, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11
Inoue, K., Suetake, T., Hayashi, F., Yokoyama, S.

Related entities 1. Rho guanine nucleotide exchange factor 11, : 1 : 1 : 230 entities Detail

Interaction partners 1. Rho guanine nucleotide exchange factor 11, : 16 interactors Detail

Experiments performed 2 experiments Detail