BMRB: 11197

Solution structure of the sixth PDZ domain of human InaD-like protein

Authors

Inoue, K., Hayashi, F., Yokoyama, S.

Assembly

InaD-like protein

Entity

1. InaD-like protein (polymer, Thiol state: not present), 117 monomers, 12314.85 Da Detail

GSSGSSGPNF SHWGPPRIVE IFREPNVSLG ISIVGGQTVI KRLKNGEELK GIFIKQVLED SPAGKTNALK TGDKILEVSG VDLQNASHSE AVEAIKNAGN PVVFIVQSLS STPRVIP

Formula weight

12314.85 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 94.9 %, Completeness (bb): 93.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (1252 of 1319)	95.3 % (654 of 686)	94.7 % (485 of 512)	93.4 % (113 of 121)
Backbone	93.4 % (641 of 686)	93.7 % (224 of 239)	93.5 % (316 of 338)	92.7 % (101 of 109)
Sidechain	96.5 % (711 of 737)	96.2 % (430 of 447)	96.8 % (269 of 278)	100.0 % (12 of 12)
Aromatic	95.0 % (57 of 60)	96.7 % (29 of 30)	93.1 % (27 of 29)	100.0 % (1 of 1)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. PDZ domain

GSSGSSGPNF SHWGPPRIVE IFREPNVSLG ISIVGGQTVI KRLKNGEELK GIFIKQVLED SPAGKTNALK TGDKILEVSG VDLQNASHSE AVEAIKNAGN PVVFIVQSLS STPRVIP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.19mM U-15N, 13C-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.19 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EHR, Strand ID: A Detail

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Release date

2011-07-19

Citation

Solution structure of the sixth PDZ domain of human InaD-like protein
Inoue, K., Hayashi, F., Yokoyama, S.

Related entities 1. InaD-like protein, : 1 : 2 : 128 entities Detail

Interaction partners 1. InaD-like protein, : 42 interactors Detail

Experiments performed 2 experiments Detail