BMRB: 11199

Solution structure of the PDZ domain of Enigma homologue protein

Authors

Qin, X., Tochio, N., Kigawa, T., Hayashi, F., Yokoyama, S.

Assembly

Enigma homologue protein

Entity

1. Enigma homologue protein (polymer, Thiol state: all free), 103 monomers, 10298.47 Da Detail

GSSGSSGSVS LVGPAPWGFR LQGGKDFNMP LTISSLKDGG KASQAHVRIG DVVLSIDGIS AQGMTHLEAQ NKIKACTGSL NMTLQRASAA AKSEPVSSGP SSG

Formula weight

10298.47 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamic

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 96.8 %, Completeness (bb): 96.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.8 % (1044 of 1079)	96.1 % (535 of 557)	97.3 % (404 of 415)	98.1 % (105 of 107)
Backbone	96.5 % (587 of 608)	96.3 % (208 of 216)	96.3 % (283 of 294)	98.0 % (96 of 98)
Sidechain	97.0 % (542 of 559)	95.9 % (327 of 341)	98.6 % (206 of 209)	100.0 % (9 of 9)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (19 of 19)	100.0 % (1 of 1)
Methyl	97.1 % (101 of 104)	94.2 % (49 of 52)	100.0 % (52 of 52)

1. PDZ domain

GSSGSSGSVS LVGPAPWGFR LQGGKDFNMP LTISSLKDGG KASQAHVRIG DVVLSIDGIS AQGMTHLEAQ NKIKACTGSL NMTLQRASAA AKSEPVSSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 1.06mM 13C, 15N-labeled {protein;} 20mM {PiNa(pH6.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.06 mM
2	PiNa	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.37 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WF7, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the PDZ domain of Enigma homologue protein
Qin, X., Tochio, N., Kigawa, T., Hayashi, F., Yokoyama, S.

Related entities 1. Enigma homologue protein, : 1 : 126 entities Detail

Interaction partners 1. Enigma homologue protein, : 8 interactors Detail

Experiments performed 2 experiments Detail