BMRB: 11203

Solution structure of the core domain of calcyclin binding protein; siah-interacting protein (SIP)

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Calcyclin-binding protein

Entity

1. Calcyclin-binding protein (polymer, Thiol state: all free), 127 monomers, 14058.86 Da Detail

GSSGSSGVVA PITTGYTVKI SNYGWDQSDK FVKIYITLTG VHQVPTENVQ VHFTERSFDL LVKNLNGKSY SMIVNNLLKP ISVEGSSKKV KTDTVLILCR KKVENTRWDY LTQVEKECKE KSGPSSG

Formula weight

14058.86 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 96.1 %, Completeness (bb): 95.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.1 % (1425 of 1483)	95.3 % (733 of 769)	97.6 % (564 of 578)	94.1 % (128 of 136)
Backbone	95.4 % (719 of 754)	93.8 % (244 of 260)	97.0 % (360 of 371)	93.5 % (115 of 123)
Sidechain	96.9 % (820 of 846)	96.1 % (489 of 509)	98.1 % (318 of 324)	100.0 % (13 of 13)
Aromatic	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (49 of 49)	100.0 % (2 of 2)
Methyl	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (74 of 74)

1. CS domain

GSSGSSGVVA PITTGYTVKI SNYGWDQSDK FVKIYITLTG VHQVPTENVQ VHFTERSFDL LVKNLNGKSY SMIVNNLLKP ISVEGSSKKV KTDTVLILCR KKVENTRWDY LTQVEKECKE KSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.98mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	CS domain	[U-13C; U-15N]	protein	0.98 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.76 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1X5M, Strand ID: A Detail

---

Release date

2011-07-20

Citation

Solution structure of the core domain of calcyclin binding protein; siah-interacting protein (SIP)
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Calcyclin-binding protein, : 1 : 23 entities Detail

Interaction partners 1. Calcyclin-binding protein, : 113 interactors Detail

Experiments performed 2 experiments Detail