BMRB: 11205

Solution structure of the first and second zf-C2H2 domain of Zinc finger protein 435

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Zinc finger protein 435

Entity

1. ADK lid domain (polymer, Thiol state: all other bound), 77 monomers, 8657.368 Da Detail

GSSGSSGRSE WQQRERRRYK CDECGKSFSH SSDLSKHRRT HTGEKPYKCD ECGKAFIQRS HLIGHHRVHT GSGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

8788.187 Da

Max. entity weight

8657.368 Da

Entity Connection

na 12 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS16:SG	2:ZN1:ZN
4	na	sing	1:CYS24:SG	2:ZN1:ZN
5	na	sing	1:HIS37:ND1	2:ZN1:ZN
6	na	sing	1:HIS29:ND1	2:ZN1:ZN
7	na	sing	1:HIS33:ND1	2:ZN1:ZN
8	na	sing	1:HIS41:ND1	2:ZN1:ZN
9	na	sing	1:CYS49:SG	2:ZN1:ZN
10	na	sing	1:CYS52:SG	2:ZN1:ZN
11	na	sing	1:HIS65:ND1	2:ZN1:ZN
12	na	sing	1:HIS69:ND1	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 92.0 %, Completeness (bb): 90.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.0 % (790 of 859)	89.9 % (411 of 457)	96.0 % (310 of 323)	87.3 % (69 of 79)
Backbone	90.8 % (416 of 458)	87.7 % (142 of 162)	94.6 % (209 of 221)	86.7 % (65 of 75)
Sidechain	93.8 % (439 of 468)	91.2 % (269 of 295)	98.2 % (166 of 169)	100.0 % (4 of 4)
Aromatic	96.1 % (73 of 76)	94.7 % (36 of 38)	97.3 % (36 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)

1. ADK lid domain

GSSGSSGRSE WQQRERRRYK CDECGKSFSH SSDLSKHRRT HTGEKPYKCD ECGKAFIQRS HLIGHHRVHT GSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.27mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.01mM ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	ADK_lid domain	[U-13C; U-15N]	protein	1.27 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.01 mM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.12 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2COT, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the first and second zf-C2H2 domain of Zinc finger protein 435
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. ADK lid domain, : 1 : 161 entities Detail

Interaction partners 1. ADK lid domain, : 2 interactors Detail

Experiments performed 2 experiments Detail