BMRB: 11206

Solution structure of the fourth PDZ domain of Glutamate receptor interacting protein 2

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

Glutamate receptor interacting protein 2

Entity

1. Glutamate receptor interacting protein 2 (polymer, Thiol state: all free), 112 monomers, 11443.59 Da Detail

GSSGSSGGGQ IVHTETTEVV LCGDPLSGFG LQLQGGIFAT ETLSSPPLVC FIEPDSPAER CGLLQVGDRV LSINGIATED GTMEEANQLL RDAALAHKVV LEVEFDSGPS SG

Formula weight

11443.59 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 94.8 %, Completeness (bb): 92.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.8 % (1111 of 1172)	94.6 % (566 of 598)	95.7 % (441 of 461)	92.0 % (104 of 113)
Backbone	92.9 % (613 of 660)	92.3 % (216 of 234)	93.8 % (300 of 320)	91.5 % (97 of 106)
Sidechain	97.0 % (590 of 608)	96.2 % (350 of 364)	98.3 % (233 of 237)	100.0 % (7 of 7)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)
Methyl	98.5 % (134 of 136)	97.1 % (66 of 68)	100.0 % (68 of 68)

1. PDZ domain

GSSGSSGGGQ IVHTETTEVV LCGDPLSGFG LQLQGGIFAT ETLSSPPLVC FIEPDSPAER CGLLQVGDRV LSINGIATED GTMEEANQLL RDAALAHKVV LEVEFDSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.95mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	0.95 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5R, Strand ID: A Detail

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Release date

2011-07-20

Citation

Tandem PDZ repeats in glutamate receptor-interacting proteins have a novel mode of PDZ domain-mediated target binding
Qin, X., Hayashi, F., Yokoyama, S.
Nat. Struct. Biol. (2003), 10, 972-978, PubMed 14555997 , DOI 10.1038/nsb992 , Abstract

Related entities 1. Glutamate receptor interacting protein 2, : 1 : 68 entities Detail

Interaction partners 1. Glutamate receptor interacting protein 2, : 3 interactors Detail

Experiments performed 2 experiments Detail