BMRB: 11208

Solution structure of the second fn3 domain of Eph receptor A8 protein

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Ephrin type-A receptor 8

Entity

1. Ephrin type-A receptor 8 (polymer, Thiol state: all free), 111 monomers, 11948.20 Da Detail

GSSGSSGQAA PSQVVVIRQE RAGQTSVSLL WQEPEQPNGI ILEYEIKYYE KDKEMQSYST LKAVTTRATV SGLKPGTRYV FQVRARTSAG CGRFSQAMEV ETGKPSGPSS G

Formula weight

11948.2 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 94.3 %, Completeness (bb): 92.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.3 % (1167 of 1238)	93.2 % (603 of 647)	96.0 % (456 of 475)	93.1 % (108 of 116)
Backbone	92.8 % (607 of 654)	91.2 % (208 of 228)	94.1 % (302 of 321)	92.4 % (97 of 105)
Sidechain	95.0 % (649 of 683)	93.6 % (392 of 419)	97.2 % (246 of 253)	100.0 % (11 of 11)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (35 of 35)	100.0 % (1 of 1)
Methyl	96.2 % (100 of 104)	96.2 % (50 of 52)	96.2 % (50 of 52)

1. fn3 domain

GSSGSSGQAA PSQVVVIRQE RAGQTSVSLL WQEPEQPNGI ILEYEIKYYE KDKEMQSYST LKAVTTRATV SGLKPGTRYV FQVRARTSAG CGRFSQAMEV ETGKPSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.42mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1.42 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.83 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5L, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the second fn3 domain of Eph receptor A8 protein
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Ephrin type-A receptor 8, : 1 : 315 entities Detail

Interaction partners 1. Ephrin type-A receptor 8, : 9 interactors Detail

Experiments performed 2 experiments Detail