BMRB: 11209

Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

SH3 domain GRB2-like protein B1

Entity

1. SH3 domain GRB2-like protein B1 (polymer, Thiol state: not present), 81 monomers, 8613.400 Da Detail

GSSGSSGLND LKESSNNRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG NQKGKVPITY LELLNSGPSS G

Formula weight

8613.4 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 94.4 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (830 of 879)	94.7 % (429 of 453)	96.8 % (328 of 339)	83.9 % (73 of 87)
Backbone	91.5 % (441 of 482)	90.5 % (153 of 169)	95.3 % (223 of 234)	82.3 % (65 of 79)
Sidechain	97.7 % (458 of 469)	97.2 % (276 of 284)	98.3 % (174 of 177)	100.0 % (8 of 8)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (22 of 22)	100.0 % (1 of 1)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. SH3 domain

GSSGSSGLND LKESSNNRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG NQKGKVPITY LELLNSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.52mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.52 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X43, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the SH3 domain of Endophilin B1 (Sh3g1b1)
Qin, X., Hayashi, F., Yokoyama, S.

Related entities 1. SH3 domain GRB2-like protein B1, : 1 : 79 entities Detail

Interaction partners 1. SH3 domain GRB2-like protein B1, : 12 interactors Detail

Experiments performed 2 experiments Detail