BMRB: 11211

Solution structure of the third ig-like domain of Myosin-dinding protein C, slow-type

Authors

Qin, X., Kurosaki, C., Hayashi, F., Yoshida, M., Yokoyama, S.

Assembly

Myosin-binding protein C, slow-type

Entity

1. Myosin-binding protein C, slow-type (polymer, Thiol state: all free), 103 monomers, 10969.13 Da Detail

GSSGSSGIMV TKQLEDTTAY CGERVELECE VSEDDANVKW FKNGEEIIPG PKSRYRIRVE GKKHILIIEG ATKADAAEYS VMTTGGQSSA KLSVDLKSGP SSG

Formula weight

10969.13 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 96.4 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1083 of 1124)	96.2 % (562 of 584)	97.2 % (423 of 435)	93.3 % (98 of 105)
Backbone	94.9 % (581 of 612)	94.4 % (203 of 215)	96.0 % (285 of 297)	93.0 % (93 of 100)
Sidechain	97.8 % (590 of 603)	97.3 % (359 of 369)	98.7 % (226 of 229)	100.0 % (5 of 5)
Aromatic	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (24 of 24)	100.0 % (1 of 1)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. IG-like domain

GSSGSSGIMV TKQLEDTTAY CGERVELECE VSEDDANVKW FKNGEEIIPG PKSRYRIRVE GKKHILIIEG ATKADAAEYS VMTTGGQSSA KLSVDLKSGP SSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	IG-like domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.59 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X44, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the third ig-like domain of Myosin-dinding protein C, slow-type
Qin, X., Kurosaki, C., Hayashi, F., Yoshida, M., Yokoyama, S.

Related entities 1. Myosin-binding protein C, slow-type, : 1 : 2 : 349 entities Detail

Interaction partners 1. Myosin-binding protein C, slow-type, : 6 interactors Detail

Experiments performed 2 experiments Detail