BMRB: 11212

Solution structure of the first ig-like domain of Myosin-binding protein C, slow-type

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Myosin-binding protein C, slow-type

Entity

1. Myosin-binding protein C, slow-type (polymer, Thiol state: all free), 126 monomers, 14040.74 Da Detail

GSSGSSGILF IEKPQGGTVK VGEDITFIAK VKAEDLLRKP TIKWFKGKWM DLASKAGKHL QLKETFERHS RVYTFEMQII KAKDNFAGNY RCEVTYKDKF DSCSFDLEVH ESTGTTPNID SGPSSG

Formula weight

14040.74 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 94.7 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (1399 of 1478)	94.4 % (728 of 771)	95.5 % (551 of 577)	92.3 % (120 of 130)
Backbone	93.7 % (701 of 748)	94.6 % (246 of 260)	93.7 % (343 of 366)	91.8 % (112 of 122)
Sidechain	95.6 % (807 of 844)	94.3 % (482 of 511)	97.5 % (317 of 325)	100.0 % (8 of 8)
Aromatic	98.6 % (138 of 140)	98.6 % (69 of 70)	98.5 % (67 of 68)	100.0 % (2 of 2)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. IG domain

GSSGSSGILF IEKPQGGTVK VGEDITFIAK VKAEDLLRKP TIKWFKGKWM DLASKAGKHL QLKETFERHS RVYTFEMQII KAKDNFAGNY RCEVTYKDKF DSCSFDLEVH ESTGTTPNID SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.32mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	IG domain	[U-13C; U-15N]	protein	1.32 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DAV, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the first ig-like domain of Myosin-binding protein C, slow-type
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Myosin-binding protein C, slow-type, : 1 : 2 : 52 entities Detail

Interaction partners 1. Myosin-binding protein C, slow-type, : 3 interactors Detail

Experiments performed 2 experiments Detail