BMRB: 11214

Solution structure of the SH3 domain of Fyn-related kinase

Authors

Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Assembly

SH3 domain of Fyn-related kinase

Entity

1. SH3 domain of Fyn-related kinase (polymer, Thiol state: not present), 77 monomers, 8324.934 Da Detail

GSSGSSGQYF VALFDYQART AEDLSFRAGD KLQVLDTSHE GWWLARHLEK KGTGLGQQLQ GYIPSNYVAE DSGPSSG

Formula weight

8324.934 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 95.3 %, Completeness (bb): 95.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (815 of 855)	94.3 % (416 of 441)	96.7 % (319 of 330)	95.2 % (80 of 84)
Backbone	95.0 % (435 of 458)	93.3 % (152 of 163)	96.4 % (212 of 220)	94.7 % (71 of 75)
Sidechain	95.9 % (444 of 463)	95.0 % (264 of 278)	97.2 % (171 of 176)	100.0 % (9 of 9)
Aromatic	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (45 of 45)	100.0 % (2 of 2)
Methyl	93.9 % (62 of 66)	93.9 % (31 of 33)	93.9 % (31 of 33)

1. SH3 domain

GSSGSSGQYF VALFDYQART AEDLSFRAGD KLQVLDTSHE GWWLARHLEK KGTGLGQQLQ GYIPSNYVAE DSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.17mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.17 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.97 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D8J, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the SH3 domain of Fyn-related kinase
Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Related entities 1. SH3 domain of Fyn-related kinase, : 1 : 2 : 148 entities Detail

Experiments performed 2 experiments Detail