BMRB: 11218

Solution structure of the CIDE-N domain of human cell death activator CIDE-A

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

CIDE-N domain of human cell death activator CIDE-A

Entity

1. CIDE-N domain of human cell death activator CIDE-A (polymer, Thiol state: not present), 91 monomers, 9723.772 Da Detail

GSSGSSGPAR PFRVSNHDRS SRRGVMASSL QELISKTLDA LVIATGLVTL VLEEDGTVVD TEEFFQTLGD NTHFMILEKG QKWMPSGPSS G

Formula weight

9723.772 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 95.3 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (952 of 999)	94.4 % (486 of 515)	96.9 % (379 of 391)	93.5 % (87 of 93)
Backbone	93.9 % (505 of 538)	91.5 % (172 of 188)	95.8 % (252 of 263)	93.1 % (81 of 87)
Sidechain	97.0 % (526 of 542)	96.0 % (314 of 327)	98.6 % (206 of 209)	100.0 % (6 of 6)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (29 of 29)	100.0 % (1 of 1)
Methyl	100.0 % (98 of 98)	100.0 % (49 of 49)	100.0 % (49 of 49)

1. CIDE-N domain

GSSGSSGPAR PFRVSNHDRS SRRGVMASSL QELISKTLDA LVIATGLVTL VLEEDGTVVD TEEFFQTLGD NTHFMILEKG QKWMPSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.10mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CIDE-N domain	[U-13C; U-15N]	protein	1.10 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.14 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.14 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EEL, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the CIDE-N domain of human cell death activator CIDE-A
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. CIDE-N domain of human cell death activator CIDE-A, : 1 : 4 : 36 entities Detail

Interaction partners 1. CIDE-N domain of human cell death activator CIDE-A, : 3 interactors Detail

Experiments performed 2 experiments Detail