BMRB: 11221

Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1

Authors

Qin, X., Suetake, T., Hayashi, F., Yokoyama, S.

Assembly

Ig-like domain of Obscurin-like protein 1

Entity

1. Ig-like domain of Obscurin-like protein 1 (polymer, Thiol state: all free), 112 monomers, 11754.78 Da Detail

GSSGSSGTAR LVAGLEDVQV YDGEDAVFSL DLSTIIQGTW FLNGEELKSN EPEGQVEPGA LRYRIEQKGL QHRLILHAVK HQDSGALVGF SCPGVQDSAA LTIQESSGPS SG

Formula weight

11754.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamic

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 96.5 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1168 of 1210)	96.1 % (599 of 623)	97.2 % (455 of 468)	95.8 % (114 of 119)
Backbone	95.3 % (633 of 664)	94.5 % (222 of 235)	96.0 % (308 of 321)	95.4 % (103 of 108)
Sidechain	97.7 % (628 of 643)	97.2 % (377 of 388)	98.4 % (240 of 244)	100.0 % (11 of 11)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (34 of 34)	100.0 % (1 of 1)
Methyl	99.2 % (123 of 124)	98.4 % (61 of 62)	100.0 % (62 of 62)

1. Ig-like domain

GSSGSSGTAR LVAGLEDVQV YDGEDAVFSL DLSTIIQGTW FLNGEELKSN EPEGQVEPGA LRYRIEQKGL QHRLILHAVK HQDSGALVGF SCPGVQDSAA LTIQESSGPS SG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.10mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ig-like domain	[U-13C; U-15N]	protein	1.10 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.7 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E6Q, Strand ID: A Detail

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Release date

2011-07-20

Citation

Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1
Qin, X., Suetake, T., Hayashi, F., Yokoyama, S.

Related entities 1. Ig-like domain of Obscurin-like protein 1, : 1 : 70 entities Detail

Interaction partners 1. Ig-like domain of Obscurin-like protein 1, : 16 interactors Detail

Experiments performed 2 experiments Detail