BMRB: 11223

Solution structure of the Ig-like domain of human Leucine-rich repeat-containing protein 4

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Leucine-rich repeat-containing protein 4

Entity

1. Leucine-rich repeat-containing protein 4 (polymer, Thiol state: all free), 103 monomers, 10750.98 Da Detail

GSSGSSGPFI MDAPRDLNIS EGRMAELKCR TPPMSSVKWL LPNGTVLSHA SRHPRISVLN DGTLNFSHVL LSDTGVYTCM VTNVAGNSNA SAYLNVSSGP SSG

Formula weight

10750.98 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 95.8 %, Completeness (bb): 94.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.8 % (1051 of 1097)	95.7 % (539 of 563)	97.0 % (416 of 429)	91.4 % (96 of 105)
Backbone	94.0 % (568 of 604)	93.3 % (195 of 209)	95.7 % (286 of 299)	90.6 % (87 of 96)
Sidechain	98.0 % (574 of 586)	97.2 % (344 of 354)	99.1 % (221 of 223)	100.0 % (9 of 9)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (29 of 29)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. IG-like domain

GSSGSSGPFI MDAPRDLNIS EGRMAELKCR TPPMSSVKWL LPNGTVLSHA SRHPRISVLN DGTLNFSHVL LSDTGVYTCM VTNVAGNSNA SAYLNVSSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.26mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3, 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	IG-like domain	[U-13C; U-15N]	protein	1.26 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.6 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DL9, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the Ig-like domain of human Leucine-rich repeat-containing protein 4
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Leucine-rich repeat-containing protein 4, : 1 : 4 : 562 entities Detail

Interaction partners 1. Leucine-rich repeat-containing protein 4, : 3 interactors Detail

Experiments performed 2 experiments Detail