BMRB: 11224

Solution structure of the SH3 domain from Phospholipase C, gamma 2

Authors

Qin, X., Nagashima, T., Hayahsi, F., Yokoyama, S.

Assembly

Phospholipase C, gamma 2

Entity

1. Phospholipase C, gamma 2 (polymer, Thiol state: all free), 69 monomers, 7564.211 Da Detail

GSSGSSGRTV KALYDYKAKR SDELTFCRGA LIHNVSKEPG GWWKGDYGTR IQQYFPSNYV EDISGPSSG

Formula weight

7564.211 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 94.6 %, Completeness (bb): 91.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.6 % (735 of 777)	95.3 % (386 of 405)	95.0 % (285 of 300)	88.9 % (64 of 72)
Backbone	91.7 % (374 of 408)	92.4 % (134 of 145)	92.4 % (182 of 197)	87.9 % (58 of 66)
Sidechain	97.4 % (417 of 428)	96.9 % (252 of 260)	98.1 % (159 of 162)	100.0 % (6 of 6)
Aromatic	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (42 of 42)	100.0 % (2 of 2)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. SH3 domain

GSSGSSGRTV KALYDYKAKR SDELTFCRGA LIHNVSKEPG GWWKGDYGTR IQQYFPSNYV EDISGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.06mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.06 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.56 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EQI, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the SH3 domain from Phospholipase C, gamma 2
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Phospholipase C, gamma 2, : 1 : 4 : 226 entities Detail

Interaction partners 1. Phospholipase C, gamma 2, : 29 interactors Detail

Experiments performed 2 experiments Detail