BMRB: 11225

Solution structure of the SH3 domain of human SLIT-ROBO Rho GTPase-activating protein 2

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

SLIT-ROBO Rho GTPase-activating protein 2

Entity

1. SLIT-ROBO Rho GTPase-activating protein 2 (polymer, Thiol state: not present), 72 monomers, 7767.426 Da Detail

GSSGSSGEPI EAIAKFDYVG RTARELSFKK GASLLLYQRA SDDWWEGRHN GIDGLIPHQY IVVQDTSGPS SG

Formula weight

7767.426 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 94.5 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (754 of 798)	94.2 % (389 of 413)	95.5 % (296 of 310)	92.0 % (69 of 75)
Backbone	93.0 % (396 of 426)	92.7 % (140 of 151)	93.7 % (193 of 206)	91.3 % (63 of 69)
Sidechain	96.1 % (417 of 434)	95.0 % (249 of 262)	97.6 % (162 of 166)	100.0 % (6 of 6)
Aromatic	98.7 % (75 of 76)	100.0 % (38 of 38)	97.2 % (35 of 36)	100.0 % (2 of 2)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. SH3 domain

GSSGSSGEPI EAIAKFDYVG RTARELSFKK GASLLLYQRA SDDWWEGRHN GIDGLIPHQY IVVQDTSGPS SG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.24mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3, 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.24 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.33 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DL8, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the SH3 domain of human SLIT-ROBO Rho GTPase-activating protein 2
Qin, X., Hayashi, F., Yokoyama, S.

Related entities 1. SLIT-ROBO Rho GTPase-activating protein 2, : 1 : 158 entities Detail

Interaction partners 1. SLIT-ROBO Rho GTPase-activating protein 2, : 23 interactors Detail

Experiments performed 2 experiments Detail