BMRB: 11226

Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2

Authors

Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Assembly

MAGUK p55 subfamily member 2

Entity

1. MAGUK p55 subfamily member 2 (polymer, Thiol state: not present), 91 monomers, 9282.546 Da Detail

GSSGSSGRMV GIRKTAGEHL GVTFRVEGGE LVIARILHGG MVAQQGLLHV GDIIKEVNGQ PVGSDPRALQ ELLRNASGSV ILKILSGPSS G

Formula weight

9282.546 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 97.4 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.4 % (953 of 978)	96.6 % (489 of 506)	98.7 % (373 of 378)	96.8 % (91 of 94)
Backbone	96.9 % (523 of 540)	95.4 % (187 of 196)	98.0 % (251 of 256)	96.6 % (85 of 88)
Sidechain	98.2 % (503 of 512)	97.4 % (302 of 310)	99.5 % (195 of 196)	100.0 % (6 of 6)
Aromatic	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. PDZ domain

GSSGSSGRMV GIRKTAGEHL GVTFRVEGGE LVIARILHGG MVAQQGLLHV GDIIKEVNGQ PVGSDPRALQ ELLRNASGSV ILKILSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.62 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.62 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.62 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E7K, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2
Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Related entities 1. MAGUK p55 subfamily member 2, : 1 : 2 : 188 entities Detail

Interaction partners 1. MAGUK p55 subfamily member 2, : 8 interactors Detail

Experiments performed 2 experiments Detail