BMRB: 11227

Solution structure of the second fn3 domain from human Ephrin type-B receptor 4

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

Ephrin type-B receptor 4

Entity

1. Ephrin type-B receptor 4 (polymer, Thiol state: not present), 109 monomers, 11442.42 Da Detail

GSSGSSGPPA VSDIRVTRSS PSSLSLAWAV PRAPSGAVLD YEVKYHEKGA EGPSSVRFLK TSENRAELRG LKRGASYLVQ VRARSEAGYG PFGQEHHSQT QLDSGPSSG

Formula weight

11442.42 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 93.5 %, Completeness (bb): 91.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.5 % (1096 of 1172)	93.5 % (571 of 611)	94.7 % (430 of 454)	88.8 % (95 of 107)
Backbone	91.2 % (582 of 638)	91.0 % (203 of 223)	92.4 % (290 of 314)	88.1 % (89 of 101)
Sidechain	96.0 % (605 of 630)	94.8 % (368 of 388)	97.9 % (231 of 236)	100.0 % (6 of 6)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (37 of 37)	100.0 % (1 of 1)
Methyl	97.9 % (92 of 94)	95.7 % (45 of 47)	100.0 % (47 of 47)

1. FN3 domain

GSSGSSGPPA VSDIRVTRSS PSSLSLAWAV PRAPSGAVLD YEVKYHEKGA EGPSSVRFLK TSENRAELRG LKRGASYLVQ VRARSEAGYG PFGQEHHSQT QLDSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.80mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]	protein	0.80 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.58 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E7H, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the second fn3 domain from human Ephrin type-B receptor 4
Qin, X., Hayashi, F., Yokoyama, S.

Related entities 1. Ephrin type-B receptor 4, : 1 : 2 : 226 entities Detail

Interaction partners 1. Ephrin type-B receptor 4, : 16 interactors Detail

Experiments performed 2 experiments Detail