BMRB: 11229

Solution structure of the Ig-like domain (714-804) from human Obscurin-like protein 1

Authors

Qin, X., Suetake, T., Hayashi, F., Yokoyama, S.

Assembly

Obscurin-like protein 1

Entity

1. Obscurin-like protein 1 (polymer, Thiol state: all free), 104 monomers, 11244.45 Da Detail

GSSGSSGPVH ILSPQDKVSL TFTTSERVVL TCELSRVDFP ATWYKDGQKV EESELLVVKM DGRKHRLILP EAKVQDSGEF ECRTEGVSAF FGVTVQDPSG PSSG

Formula weight

11244.45 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamic

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 97.1 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.1 % (1125 of 1159)	96.8 % (581 of 600)	97.8 % (446 of 456)	95.1 % (98 of 103)
Backbone	95.8 % (586 of 612)	94.8 % (201 of 212)	96.7 % (292 of 302)	94.9 % (93 of 98)
Sidechain	98.4 % (631 of 641)	97.9 % (380 of 388)	99.2 % (246 of 248)	100.0 % (5 of 5)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (38 of 38)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. Ig-like domain

GSSGSSGPVH ILSPQDKVSL TFTTSERVVL TCELSRVDFP ATWYKDGQKV EESELLVVKM DGRKHRLILP EAKVQDSGEF ECRTEGVSAF FGVTVQDPSG PSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.17mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ig-like domain	[U-13C; U-15N]	protein	1.17 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.84 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E6P, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the Ig-like domain (714-804) from human Obscurin-like protein 1
Qin, X., Suetake, T., Hayashi, F., Yokoyama, S.

Related entities 1. Obscurin-like protein 1, : 1 : 132 entities Detail

Interaction partners 1. Obscurin-like protein 1, : 16 interactors Detail

Experiments performed 2 experiments Detail