BMRB: 11230

Solution structure of the 6th Ig-like domain from human KIAA1556

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Obscurin

Entity

1. Obscurin (polymer, Thiol state: all free), 103 monomers, 10778.99 Da Detail

GSSGSSGPVR FQEALKDLEV LEGGAATLRC VLSSVAAPVK WCYGNNVLRP GDKYSLRQEG AMLELVVRNL RPQDSGRYSC SFGDQTTSAT LTVTALPSGP SSG

Formula weight

10778.99 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 93.1 %, Completeness (bb): 90.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (1030 of 1106)	92.3 % (527 of 571)	94.7 % (407 of 430)	91.4 % (96 of 105)
Backbone	90.9 % (551 of 606)	89.2 % (189 of 212)	92.3 % (274 of 297)	90.7 % (88 of 97)
Sidechain	95.6 % (565 of 591)	94.2 % (338 of 359)	97.8 % (219 of 224)	100.0 % (8 of 8)
Aromatic	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (27 of 27)	100.0 % (1 of 1)
Methyl	98.2 % (110 of 112)	96.4 % (54 of 56)	100.0 % (56 of 56)

1. Ig-like domain, residues 8-97

GSSGSSGPVR FQEALKDLEV LEGGAATLRC VLSSVAAPVK WCYGNNVLRP GDKYSLRQEG AMLELVVRNL RPQDSGRYSC SFGDQTTSAT LTVTALPSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 0.39mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ig-like domain, residues 8-97	[U-13C; U-15N]	protein	0.39 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.96 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E7B, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the 6th Ig-like domain from human KIAA1556
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Obscurin, : 1 : 1 : 538 entities Detail

Interaction partners 1. Obscurin, : 21 interactors Detail

Experiments performed 2 experiments Detail