BMRB: 11231

Solution structure of the PKD domain from KIAA 1837 protein

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

KIAA1837 protein

Entity

1. KIAA1837 protein (polymer, Thiol state: not present), 102 monomers, 10578.29 Da Detail

GSSGSSGQAD AGPDKELTLP VDSTTLDGSK SSDDQKIISY LWEKTQGPDG VQLENANSSV ATVTGLQVGT YVFTLTVKDE RNLQSQSSVN VIVKEESGPS SG

Formula weight

10578.29 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 96.7 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.7 % (1046 of 1082)	97.5 % (542 of 556)	97.1 % (404 of 416)	90.9 % (100 of 110)
Backbone	94.9 % (573 of 604)	95.7 % (202 of 211)	95.9 % (283 of 295)	89.8 % (88 of 98)
Sidechain	98.6 % (561 of 569)	98.6 % (340 of 345)	98.6 % (209 of 212)	100.0 % (12 of 12)
Aromatic	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (18 of 18)	100.0 % (1 of 1)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. PKD domain

GSSGSSGQAD AGPDKELTLP VDSTTLDGSK SSDDQKIISY LWEKTQGPDG VQLENANSSV ATVTGLQVGT YVFTLTVKDE RNLQSQSSVN VIVKEESGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.13mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PKD domain	[U-13C; U-15N]	protein	1.13 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.84 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YRL, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the PKD domain from KIAA 1837 protein
Qin, X., Hayashi, F., Yokoyama, S.

Related entities 1. KIAA1837 protein, : 1 : 2 : 44 entities Detail

Interaction partners 1. KIAA1837 protein, : 4 interactors Detail

Experiments performed 2 experiments Detail