BMRB: 11232

Solution structure of the PKD domain (329-428) from human KIAA0319

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Protein KIAA0319

Entity

1. Protein KIAA0319 (polymer, Thiol state: not present), 113 monomers, 12024.22 Da Detail

GSSGSSGPRT VKELTVSAGD NLIITLPDNE VELKAFVAPA PPVETTYNYE WNLISHPTDY QGEIKQGHKQ TLNLSQLSVG LYVFKVTVSS ENAFGEGFVN VTVKPARSGP SSG

Formula weight

12024.22 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 94.2 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.2 % (1187 of 1260)	93.2 % (604 of 648)	95.8 % (474 of 495)	93.2 % (109 of 117)
Backbone	92.4 % (612 of 662)	90.0 % (206 of 229)	94.2 % (309 of 328)	92.4 % (97 of 105)
Sidechain	96.1 % (673 of 700)	95.0 % (398 of 419)	97.8 % (263 of 269)	100.0 % (12 of 12)
Aromatic	97.8 % (90 of 92)	97.8 % (45 of 46)	97.8 % (44 of 45)	100.0 % (1 of 1)
Methyl	98.5 % (128 of 130)	98.5 % (64 of 65)	98.5 % (64 of 65)

1. PKD domain

GSSGSSGPRT VKELTVSAGD NLIITLPDNE VELKAFVAPA PPVETTYNYE WNLISHPTDY QGEIKQGHKQ TLNLSQLSVG LYVFKVTVSS ENAFGEGFVN VTVKPARSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.16mM 13C,15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PKD domain	[U-13C; U-15N]	protein	1.16 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.89 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E7M, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the PKD domain (329-428) from human KIAA0319
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Protein KIAA0319, : 1 : 26 entities Detail

Interaction partners 1. Protein KIAA0319, : 3 interactors Detail

Experiments performed 2 experiments Detail