BMRB: 11233

Solution structure of the SH3 domain of human KIAA1783 protein

Authors

Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Assembly

KIAA1783 protein

Entity

1. KIAA1783 protein (polymer, Thiol state: all free), 85 monomers, 8677.517 Da Detail

GSSGSSGSGY VIALRSYITD NCSLLSFHRG DLIKLLPVAT LEPGWQFGSA GGRSGLFPAD IVQPAAAPDF SFSKEQRSGS GPSSG

Formula weight

8677.517 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 91.6 %, Completeness (bb): 89.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (833 of 909)	91.0 % (426 of 468)	93.3 % (333 of 357)	88.1 % (74 of 84)
Backbone	89.2 % (444 of 498)	87.6 % (155 of 177)	90.9 % (220 of 242)	87.3 % (69 of 79)
Sidechain	94.4 % (456 of 483)	93.1 % (271 of 291)	96.3 % (180 of 187)	100.0 % (5 of 5)
Aromatic	91.5 % (75 of 82)	87.8 % (36 of 41)	95.0 % (38 of 40)	100.0 % (1 of 1)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. SH3 domain

GSSGSSGSGY VIALRSYITD NCSLLSFHRG DLIKLLPVAT LEPGWQFGSA GGRSGLFPAD IVQPAAAPDF SFSKEQRSGS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.15mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.15 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.57 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.57 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.13 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DLP, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the SH3 domain of human KIAA1783 protein
Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Related entities 1. KIAA1783 protein, : 1 : 2 : 43 entities Detail

Interaction partners 1. KIAA1783 protein, : 2 interactors Detail

Experiments performed 2 experiments Detail