BMRB: 11234

Solution structure of the RING domain (1-66) from tripartite motif-containing protein 31

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Tripartite motif-containing protein 31

Entity

1. RING-type zinc finger (polymer, Thiol state: free and other bound), 73 monomers, 7855.164 Da Detail

GSSGSSGMAS GQFVNKLQEE VICPICLDIL QKPVTIDCGH NFCLKCITQI GETSCGFFKC PLCKTSVRKN AIR

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7985.982 Da

Max. entity weight

7855.164 Da

Entity Connection

na 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS23:SG	2:ZN1:ZN
2	na	sing	1:CYS26:SG	2:ZN1:ZN
3	na	sing	1:CYS43:SG	2:ZN1:ZN
4	na	sing	1:CYS46:SG	2:ZN1:ZN
5	na	sing	1:CYS38:SG	2:ZN1:ZN
6	na	sing	1:HIS40:ND1	2:ZN1:ZN
7	na	sing	1:CYS60:SG	2:ZN1:ZN
8	na	sing	1:CYS63:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 93.5 %, Completeness (bb): 89.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.5 % (773 of 827)	94.4 % (407 of 431)	94.4 % (301 of 319)	84.4 % (65 of 77)
Backbone	89.6 % (387 of 432)	90.0 % (135 of 150)	91.5 % (194 of 212)	82.9 % (58 of 70)
Sidechain	97.4 % (449 of 461)	96.8 % (272 of 281)	98.3 % (170 of 173)	100.0 % (7 of 7)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. RING-type zinc finger

GSSGSSGMAS GQFVNKLQEE VICPICLDIL QKPVTIDCGH NFCLKCITQI GETSCGFFKC PLCKTSVRKN AIR

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.16mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM ZnCl2+1mM IDA/ 90% H2O, 10%D2O

#	Name	Isotope labeling	Type	Concentration
1	RING-type zinc finger	[U-13C; U-15N]	protein	1.16 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YSL, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the RING domain (1-66) from tripartite motif-containing protein 31
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. RING-type zinc finger, : 1 : 3 : 156 entities Detail

Interaction partners 1. RING-type zinc finger, : 11 interactors Detail

Experiments performed 2 experiments Detail