BMRB: 11235

Solution structure of the 6th Ig-like domain from human Myosin-binding protein C, fast-type

Authors

Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

Myosin-binding protein C, fast-type

Entity

1. Myosin-binding protein C, fast-type (polymer, Thiol state: not present), 118 monomers, 13126.81 Da Detail

GSSGSSGTLA QPVTIREIAE PPKIRLPRHL RQTYIRKVGE QLNLVVPFQG KPRPQVVWTK GGAPLDTSRV HVRTSDFDTV FFVRQAARSD SGEYELSVQI ENMKDTATIR IRVVEKAG

Formula weight

13126.81 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 97.1 %, Completeness (bb): 96.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.1 % (1331 of 1371)	96.8 % (695 of 718)	97.6 % (520 of 533)	96.7 % (116 of 120)
Backbone	96.4 % (667 of 692)	96.6 % (229 of 237)	96.2 % (332 of 345)	96.4 % (106 of 110)
Sidechain	97.7 % (770 of 788)	96.9 % (466 of 481)	99.0 % (294 of 297)	100.0 % (10 of 10)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (37 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. Ig-like domain

GSSGSSGTLA QPVTIREIAE PPKIRLPRHL RQTYIRKVGE QLNLVVPFQG KPRPQVVWTK GGAPLDTSRV HVRTSDFDTV FFVRQAARSD SGEYELSVQI ENMKDTATIR IRVVEKAG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.04mM 13C, 15N-labeled {protein;} 20mM {d-Tris-HCl(pH7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3, 90% {H2O;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ig-like domain	[U-13C; U-15N]	protein	1.04 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.64 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2E7C, Strand ID: A Detail

---

Release date

2011-08-02

Citation

Solution structure of the 6th Ig-like domain from human Myosin-binding protein C, fast-type
Qin, X., Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. Myosin-binding protein C, fast-type, : 1 : 2 : 283 entities Detail

Interaction partners 1. Myosin-binding protein C, fast-type, : 31 interactors Detail

Experiments performed 2 experiments Detail