BMRB: 11236

Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle

Authors

Qin, X., Kurosaki, C., Yoshida, M., Hayahsi, F., Yokoyama, S.

Assembly

Myosin light chain kinase, smooth muscle

Entity

1. Myosin light chain kinase, smooth muscle (polymer, Thiol state: all free), 99 monomers, 10450.61 Da Detail

GSSGSSGMEV APSFSSVLKD CAVIEGQDFV LQCSVRGTPV PRITWLLNGQ PIQYARSTCE AGVAELHIQD ALPEDHGTYT CLAENALGQV SCSAWVTVH

Formula weight

10450.61 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 95.2 %, Completeness (bb): 93.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.2 % (1009 of 1060)	95.5 % (515 of 539)	95.2 % (397 of 417)	93.3 % (97 of 104)
Backbone	93.3 % (545 of 584)	94.1 % (190 of 202)	93.1 % (268 of 288)	92.6 % (87 of 94)
Sidechain	97.2 % (550 of 566)	96.4 % (325 of 337)	98.2 % (215 of 219)	100.0 % (10 of 10)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (34 of 34)	100.0 % (2 of 2)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. Ig domain

GSSGSSGMEV APSFSSVLKD CAVIEGQDFV LQCSVRGTPV PRITWLLNGQ PIQYARSTCE AGVAELHIQD ALPEDHGTYT CLAENALGQV SCSAWVTVH

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.13mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ig domain	[U-13C; U-15N]	protein	1.13 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.54 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.54 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.25 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YR3, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle
Qin, X., Kurosaki, C., Yoshida, M., Hayashi, F., Yokoyama, S.

Related entities 1. Myosin light chain kinase, smooth muscle, : 1 : 2 : 818 entities Detail

Interaction partners 1. Myosin light chain kinase, smooth muscle, : 19 interactors Detail

Experiments performed 2 experiments Detail