BMRB: 11238

Solution structure of the C2H2-type zinc finger domain (699-729) from zinc finger protein 473

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

Zinc finger protein 473

Entity

1. C2H2-type zinc finger (polymer, Thiol state: free and other bound), 44 monomers, 4518.981 Da Detail

GSSGSSGKKP LVCNECGKTF RQSSCLSKHQ RIHSGEKPSG PSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4584.39 Da

Max. entity weight

4518.981 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 89.8 %, Completeness (bb): 85.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.8 % (422 of 470)	90.4 % (227 of 251)	91.4 % (160 of 175)	79.5 % (35 of 44)
Backbone	85.3 % (220 of 258)	84.8 % (78 of 92)	88.0 % (110 of 125)	78.0 % (32 of 41)
Sidechain	94.4 % (235 of 249)	93.7 % (149 of 159)	95.4 % (83 of 87)	100.0 % (3 of 3)
Aromatic	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. C2H2-type zinc finger

GSSGSSGKKP LVCNECGKTF RQSSCLSKHQ RIHSGEKPSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 0.06mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM ZnCl2, 1mM {IDA;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C2H2-type zinc finger	[U-13C; U-15N]	protein	0.06 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	0.05 mM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YRH, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the C2H2-type zinc finger domain (699-729) from zinc finger protein 473
Qin, X., Hayashi, F., Yokoyama, S.

Related entities 1. C2H2-type zinc finger, : 1 : 207 entities Detail

Experiments performed 2 experiments Detail