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BMRB: 11239


11239
2YRJ
Solution structure of the C2H2-type zinc finger domain (781-813) from zinc finger protein 473
Authors
Qin, X., Izumi, K., Yoshida, M., Hayashi, F., Yokoyama, S.
Assembly
Zinc finger protein 473
Entity
1. C2H2-type zinc finger (polymer, Thiol state: all other bound), 46 monomers, 4677.028 Da Detail

GSSGSSGTGE KPYRCGECGK AFAQKANLTQ HQRIHTGEKP SGPSSG


2. ZN (non-polymer), 65.409 Da
Total weight
4742.437 Da
Max. entity weight
4677.028 Da
Entity Connection
na 4 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS15:SG2:ZN1:ZN
2nasing1:CYS18:SG2:ZN1:ZN
3nasing1:HIS31:ND12:ZN1:ZN
4nasing1:HIS35:ND12:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 91.3 %, Completeness: 91.5 %, Completeness (bb): 87.0 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All91.5 % (439 of 480)92.9 % (236 of 254)91.6 % (164 of 179)83.0 % (39 of 47)
Backbone87.0 % (235 of 270)87.8 % (86 of 98)88.4 % (114 of 129)81.4 % (35 of 43)
Sidechain96.4 % (238 of 247)96.2 % (150 of 156)96.6 % (84 of 87)100.0 % (4 of 4)
Aromatic100.0 % (26 of 26)100.0 % (13 of 13)100.0 % (13 of 13)
Methyl100.0 % (20 of 20)100.0 % (10 of 10)100.0 % (10 of 10)

1. C2H2-type zinc finger

GSSGSSGTGE KPYRCGECGK AFAQKANLTQ HQRIHTGEKP SGPSSG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.08mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM ZnCl2+1mM IDA, 90% H2O, 10%D2O


#NameIsotope labelingTypeConcentration
1C2H2-type zinc finger[U-13C; U-15N]protein1.08 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6ZnCl2natural abundancesalt0.05 mM
7IDAnatural abundancesalt1 mM
8H2Osolvent90 %
9D2Osolvent10 %

LACS Plot; CA
Referencing offset: 2.51 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 2.51 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 2.45 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2YRJ, Strand ID: A Detail

Release date
2011-08-02
Citation
Solution structure of the C2H2-type zinc finger domain (781-813) from zinc finger protein 473
Qin, X., Izumi, K., Yoshida, M., Hayashi, F., Yokoyama, S.
Related entities 1. C2H2-type zinc finger, : 1 : 171 entities Detail
More details for 172 related entities
Experiments performed 2 experiments Detail
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