BMRB: 11244

Solution structure of the first SANT domain from human nuclear receptor corepressor 1

Authors

Zhang, H., Hayahsi, F., Yokoyama, S.

Assembly

Nuclear receptor corepressor 1

Entity

1. Nuclear receptor corepressor 1 (polymer, Thiol state: all free), 61 monomers, 7160.963 Da Detail

GSSGSSGDRQ FMNVWTDHEK EIFKDKFIQH PKNFGLIASY LERKSVPDCV LYYYLTKKNE N

Formula weight

7160.963 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 95.7 %, Completeness (bb): 94.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.7 % (718 of 750)	96.2 % (379 of 394)	95.5 % (277 of 290)	93.9 % (62 of 66)
Backbone	94.8 % (343 of 362)	95.2 % (118 of 124)	94.4 % (169 of 179)	94.9 % (56 of 59)
Sidechain	96.6 % (430 of 445)	96.7 % (261 of 270)	97.0 % (163 of 168)	85.7 % (6 of 7)
Aromatic	93.5 % (86 of 92)	93.5 % (43 of 46)	93.3 % (42 of 45)	100.0 % (1 of 1)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. Sant domain

GSSGSSGDRQ FMNVWTDHEK EIFKDKFIQH PKNFGLIASY LERKSVPDCV LYYYLTKKNE N

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 293 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM 13C, 15N-labeled {protein;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Sant domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2EQR, Strand ID: A Detail

---

Release date

2011-08-02

Citation

Solution structure of the first SANT domain from human nuclear receptor corepressor 1
Zhang, H., Hayashi, F., Yokoyama, S.

Related entities 1. Nuclear receptor corepressor 1, : 1 : 4 : 66 entities Detail

Interaction partners 1. Nuclear receptor corepressor 1, : 24 interactors Detail

Experiments performed 2 experiments Detail