BMRB: 11246

Solution structure of the FHA domain of mouse Afadin 6

Authors

Hayashi, F., Hirata, Y., Guntert, P., Yokoyama, S.

Assembly

Afadin 6

Entity

1. Afadin 6 (polymer, Thiol state: all free), 120 monomers, 12980.26 Da Detail

GSSGSSGPEK LPYLVELSPD GSDSRDKPKL YRLQLSVTEV GTEKFDDNSI QLFGPGIQPH HCDLTNMDGV VTVTPRSMDA ETYVDGQRIS ETTMLQSGMR LQFGTSHVFK FVDPSGPSSG

Formula weight

12980.26 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.2 %, Completeness (bb): 99.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.2 % (1319 of 1329)	99.0 % (684 of 691)	99.8 % (518 of 519)	98.3 % (117 of 119)
Backbone	99.0 % (695 of 702)	98.4 % (240 of 244)	99.7 % (346 of 347)	98.2 % (109 of 111)
Sidechain	99.6 % (731 of 734)	99.3 % (444 of 447)	100.0 % (279 of 279)	100.0 % (8 of 8)
Aromatic	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)
Methyl	99.1 % (107 of 108)	98.1 % (53 of 54)	100.0 % (54 of 54)

1. FHA domain

GSSGSSGPEK LPYLVELSPD GSDSRDKPKL YRLQLSVTEV GTEKFDDNSI QLFGPGIQPH HCDLTNMDGV VTVTPRSMDA ETYVDGQRIS ETTMLQSGMR LQFGTSHVFK FVDPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05), Details 0.7mM FHA {domain;} {U-15N,13C;} 20mM phosphate buffer {NA;} 100mM {NaCl;} 0.02% {NaN3;} 1mMDTT

#	Name	Isotope labeling	Type	Concentration
1	FHA domain	[U-13C; U-15N]	protein	0.7 mM
2	phosphate	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.65 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WLN, Strand ID: A Detail

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Release date

2011-08-02

Citation

Solution structure of the FHA domain of mouse Afadin 6
Hayashi, F., Hirata, Y., Guntert, P., Yokoyama, S.

Related entities 1. Afadin 6, : 1 : 63 entities Detail

Interaction partners 1. Afadin 6, : 4 interactors Detail

Experiments performed 2 experiments Detail